Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97054

CNTF rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CNTF rabbit pAb from ELK Biotechnology Read more ›

CNTF rabbit pAb
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CNTF rabbit pAb

Product Description

CNTF rabbit pAb is a research-use antibody from ELK Biotechnology for studying CNTF in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010],

Additional antibody information

Specificity CNTF protein(A218) detects endogenous levels of CNTF
Validation evidence captions Western blot analysis of 1) Hela, 2)Mouse Brain Tissue, 3)Rat Brain Tissue with CNTF Rabbit pAb diluted at 1:2,000.

Key Facts

Product typePrimary Antibody Target: CNTF
Collaborator ELK Biotechnology
Partner model ES20782
Target CNTF
Research areas>>Cytokine-cytokine receptor interaction, >>JAK-STAT signaling pathway
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-1000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of CNTF
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Nervous system.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-1000
Rat
Mouse

Specifications

Target
CNTF
Also known as
CNTF; Ciliary neurotrophic factor; CNTF
Concentration
1 mg/ml
Working concentration
WB 1:500-1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthetic Peptide of CNTF
Observed band
30kD
Cellular localization
Cytoplasm.
Dilution range
WB 1:500-1000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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