Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97050

EAAT2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

EAAT2 rabbit pAb from ELK Biotechnology Read more ›

EAAT2 rabbit pAb 50µL/100µL
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EAAT2 rabbit pAb 50µL/100µL

Product Description

EAAT2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying EAAT2 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010],

Additional antibody information

Specificity EAAT2 protein(A223) detects endogenous levels of EAAT2
Validation evidence captions Western blot analysis of 1) Mouse Brain Tissue, 2)Rat Brain Tissue with EAAT2 Rabbit pAb diluted at 1:2,000.

Key Facts

Product typePrimary Antibody Target: EAAT2
Collaborator ELK Biotechnology
Partner model ES20771
Target EAAT2
Research areas>>Synaptic vesicle cycle, >>Glutamatergic synapse, >>Amyotrophic lateral sclerosis, >>Huntington disease
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of EAAT2 AA range: 370-420
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Brain cortex,Pancreas,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Rat
Mouse

Specifications

Target
EAAT2
Also known as
Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthetic Peptide of EAAT2 AA range: 370-420
Observed band
62kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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