EAAT2 rabbit pAb
EAAT2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
EAAT2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving EAAT2.
Background
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010],
Additional antibody information
| Specificity | EAAT2 protein(A223) detects endogenous levels of EAAT2 |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Mouse Brain Tissue, 2)Rat Brain Tissue with EAAT2 Rabbit pAb diluted at 1:2,000. |
Key Facts
| Target | EAAT2 |
| Also known as | Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Rat;Mouse |
| Observed band | 62kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Synaptic vesicle cycle;>>Glutamatergic synapse;>>Amyotrophic lateral sclerosis;>>Huntington disease |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthetic Peptide of EAAT2 AA range: 370-420 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Rat | — | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.