EFHD1 rabbit pAb
EFHD1 rabbit pAb from ELK Biotechnology Read more ›
Product Description
EFHD1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving EFHD1.
Background
This gene encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. The protein encoded by this gene is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. This protein has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016],
Additional antibody information
| Specificity | The antibody detects endogenous EFHD1 proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of 293T cell lysate, diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | EFHD1 |
| Also known as | EF-hand domain-containing protein D1 (EF-hand domain-containing protein 1) (Swiprosin-2) |
| Working concentration | WB: 1:1000 |
| Species reactivity | Human |
| Observed band | 27kD |
| Cellular localization | Mitochondrion inner membrane . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:1000 |
| Immunogen | Recombinant Protein of EF-hand domain-containing protein D1 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:1000 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.