Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97047

EFHD1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

EFHD1 rabbit pAb from ELK Biotechnology Read more ›

EFHD1 rabbit pAb
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EFHD1 rabbit pAb

Product Description

EFHD1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying EFHD1 in WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. The protein encoded by this gene is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. This protein has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016],

Additional antibody information

Specificity The antibody detects endogenous EFHD1 proteins.
Validation evidence captions Western blot analysis of 293T cell lysate, diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: EFHD1
Collaborator ELK Biotechnology
Partner model ES20767
Target EFHD1
Research areasAntibody Research
Reactivity Human
Applications WB
ConjugationUnconjugated
Dilution rangeWB: 1:1000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein of EF-hand domain-containing protein D1
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Eye,Heart,Hippocampus,Lung,Normal aorta,Placenta,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB: 1:1000

Specifications

Target
EFHD1
Also known as
EF-hand domain-containing protein D1 (EF-hand domain-containing protein 1) (Swiprosin-2)
Working concentration
WB: 1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WB
Immunogen
Recombinant Protein of EF-hand domain-containing protein D1
Observed band
27kD
Cellular localization
Mitochondrion inner membrane .
Dilution range
WB: 1:1000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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