EYFP rabbit pAb from ELK Biotechnology Read more ›
EYFP rabbit pAb is a research-use antibody from ELK Biotechnology for studying EYFP in WB workflows with listed reactivity for General. Specifications and supporting documents below should be reviewed when planning the experiment.
Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
| Specificity | The antibody detects EYFP and YFP tag fusion proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of EYFP recombinant protein, diluted at 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES20756 |
| Target | EYFP |
| Research areas | Antibody Research |
| Reactivity | General |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:3000-5000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of EYFP |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Species independent |
| Species | Dilution | Notes |
|---|---|---|
| Species independent | WB: 1:3000-5000 | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
