EYFP rabbit pAb
EYFP rabbit pAb from ELK Biotechnology Read more ›
Product Description
EYFP rabbit pAb is an ELK Biotechnology antibody for research workflows involving EYFP.
Background
Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Additional antibody information
| Specificity | The antibody detects EYFP and YFP tag fusion proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of EYFP recombinant protein, diluted at 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | EYFP |
| Also known as | YM3229 |
| Working concentration | WB: 1:3000-5000 |
| Species reactivity | Species independent |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:3000-5000 |
| Immunogen | Recombinant Protein of EYFP |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Species independent |
| Species | Dilution | Notes |
|---|---|---|
| Species independent | WB: 1:3000-5000 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.