FH rabbit pAb
FH rabbit pAb from ELK Biotechnology Read more ›
Product Description
FH rabbit pAb is an ELK Biotechnology antibody for research workflows involving FH.
Background
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous fumarase proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Mouse Brain, 3) HepG2, diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | FH |
| Also known as | Fumarate hydratase, mitochondrial (Fumarase) (EC 4.2.1.2) |
| Working concentration | WB: 1:1000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 50kD |
| Cellular localization | Isoform Mitochondrial: Mitochondrion .; Isoform Cytoplasmic: Cytoplasm, cytosol . Nucleus . Chromosome . Translocates to the nucleus in response to DNA damage: localizes to DNA double-strand breaks (DSBs) following phosphorylation by PRKDC. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Citrate cycle (TCA cycle);>>Pyruvate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Cushing syndrome;>>Pathways in cancer;>>Renal cell carcinoma |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:1000 |
| Immunogen | Recombinant Protein of Fumarate hydratase, mitochondrial |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:1000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.