GAL4 DNA Binding Domain rabbit pAb
GAL4 DNA Binding Domain rabbit pAb from ELK Biotechnology Read more ›
Product Description
GAL4 DNA Binding Domain rabbit pAb is an ELK Biotechnology antibody for research workflows involving GAL4 DNA Binding Domain.
Background
The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
Additional antibody information
| Specificity | The antibody detects endogenous GAL4 DNA Binding Domain protein. |
|---|---|
| Validation evidence captions | Western blot analysis of Recombinant BD protein using GAL4 DNA Binding Domain Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | GAL4 DNA Binding Domain |
| Also known as | YM3411 |
| Working concentration | WB: 1:10000-50000 |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:10000-50000 |
| Immunogen | Recombinant Protein of GAL4 DNA Binding Domain |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| GAL4 DNA Binding Domain |
| Species | Dilution | Notes |
|---|---|---|
| GAL4 DNA Binding Domain | WB: 1:10000-50000 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.