GAL4 DNA Binding Domain rabbit pAb from ELK Biotechnology Read more ›
GAL4 DNA Binding Domain rabbit pAb is a research-use antibody from ELK Biotechnology for studying GAL4 DNA Binding Domain in WB workflows. Specifications and supporting documents below should be reviewed when planning the experiment.
The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
| Specificity | The antibody detects endogenous GAL4 DNA Binding Domain protein. |
|---|---|
| Validation evidence captions | Western blot analysis of Recombinant BD protein using GAL4 DNA Binding Domain Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES20743 |
| Target | GAL4 DNA Binding Domain |
| Research areas | Antibody Research |
| Reactivity | GAL4 DNA Binding Domain:C |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:10000-50000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of GAL4 DNA Binding Domain |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| GAL4 DNA Binding Domain |
| Species | Dilution | Notes |
|---|---|---|
| GAL4 DNA Binding Domain | WB: 1:10000-50000 | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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