MeCP2 rabbit pAb
MeCP2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
MeCP2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving MeCP2.
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isofor
Additional antibody information
| Specificity | The antibody detects endogenous MeCP2 proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | MeCP2 |
| Also known as | Methyl-CpG-binding protein 2 (MeCp-2 protein) (MeCp2) |
| Working concentration | WB: 1:2000 |
| Species reactivity | Human |
| Observed band | 53kD |
| Cellular localization | Nucleus . Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:2000 |
| Immunogen | Synthetic Peptide of MeCP2 AA range: 313-363 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:2000 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.