MeCP2 rabbit pAb from ELK Biotechnology Read more ›
MeCP2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MeCP2 in WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isofor
| Specificity | The antibody detects endogenous MeCP2 proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES20671 |
| Target | MeCP2 |
| Research areas | Antibody Research |
| Reactivity | Human |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide of MeCP2 AA range: 313-363 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Present in all adult somatic tissues tested. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:2000 | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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