Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97030

PEPC rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PEPC rabbit pAb from ELK Biotechnology Read more ›

PEPC rabbit pAb
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PEPC rabbit pAb

Product Description

PEPC rabbit pAb is a research-use antibody from ELK Biotechnology for studying PEPC in WB workflows with listed reactivity for Plants. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009],

Additional antibody information

Specificity The antibody detects endogenous PEPC protein.
Validation evidence captions Western blot analysis of Maize, diluted at 1:4000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: PEPC
Collaborator ELK Biotechnology
Partner model ES20649
Target PEPC
Research areasAntibody Research
Reactivity Plants
Applications WB
ConjugationUnconjugated
Dilution rangeWB: 1:2000-5000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of PEPC
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Liver,Placenta,Stomach,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Plants
ABMIUM Validated™ WB
Species Dilution Notes
Plants WB: 1:2000-5000

Specifications

Target
PEPC
Also known as
PGC; Gastricsin; Pepsinogen C
Working concentration
WB: 1:2000-5000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Plants
Tested applications
WB
Immunogen
Synthetic Peptide of PEPC
Observed band
105-109kD
Cellular localization
extracellular space,extracellular exosome,
Dilution range
WB: 1:2000-5000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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