β Tubulin rabbit pAb
β Tubulin rabbit pAb from ELK Biotechnology Read more ›
Product Description
β Tubulin rabbit pAb is an ELK Biotechnology antibody for research workflows involving Tubulin β.
Background
tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],
Additional antibody information
| Specificity | The antibody detects endogenous β tubulin protein. |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Mouse Brain, 3) Rat Brain tissue, diluted at 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | Tubulin β |
| Also known as | TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III |
| Working concentration | WB: 1:2000-5000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 55kD |
| Cellular localization | Cytoplasm, cytoskeleton . Cell projection, growth cone . Cell projection, lamellipodium . Cell projection, filopodium . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Phagosome;>>Gap junction;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:2000-5000 |
| Immunogen | Recombinant Protein of Tubulin beta |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:2000-5000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.