Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97025

β Tubulin rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

β Tubulin rabbit pAb from ELK Biotechnology Read more ›

β Tubulin rabbit pAb
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β Tubulin rabbit pAb

Product Description

β Tubulin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Tubulin β in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],

Additional antibody information

Specificity The antibody detects endogenous β tubulin protein.
Validation evidence captions Western blot analysis of 1) Hela, 2) Mouse Brain, 3) Rat Brain tissue, diluted at 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: Tubulin β
Collaborator ELK Biotechnology
Partner model ES20589
Target Tubulin β
Research areas>>Phagosome, >>Gap junction, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Pathogenic Escherichia coli infection, >>Salmonella infection
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB: 1:2000-5000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein of Tubulin beta
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB: 1:2000-5000
Mouse
Rat

Specifications

Target
Tubulin β
Also known as
TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Working concentration
WB: 1:2000-5000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Recombinant Protein of Tubulin beta
Observed band
55kD
Cellular localization
Cytoplasm, cytoskeleton . Cell projection, growth cone . Cell projection, lamellipodium . Cell projection, filopodium .
Dilution range
WB: 1:2000-5000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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