β-Catenin rabbit pAb from ELK Biotechnology Read more ›
β-Catenin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Catenin-β in WB workflows with listed reactivity for Mouse, Rat, Rabbit. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016],
| Specificity | The antibody detects endogenous β-Catenin protein. |
|---|---|
| Validation evidence captions | Western blot analysis of 1) 3T3, 2) Rat Liver, diluted at 1:2,000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES20585 |
| Target | Catenin-β |
| Research areas | >>Rap1 signaling pathway, >>Wnt signaling pathway, >>Hippo signaling pathway, >>Focal adhesion, >>Adherens junction, >>Signaling pathways regulating pluripotency of stem cells, >>Leukocyte transendothelial migration, >>Melanogenesis, >>Thyroid hormone signaling pathway, >>Cushing syndrome, >>Alcoholic liver disease, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Bacterial invasion of epithelial cells, >>Salmonella infection, >>Hepatitis C, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Colorectal cancer, >>Endometrial cancer, >>Prostate cancer, >>Thyroid cancer, >>Basal cell carcinoma, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer, >>Arrhythmogenic right ventricular cardiomyopathy, >>Fluid shear stress and atherosclerosis |
| Reactivity | Mouse,Rat,Rabbit |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:2000-4000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of Catenin beta-1 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (PubMed:29367600). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat | |||||||
| Rabbit |
| Species | Dilution | Notes |
|---|---|---|
| Mouse | WB: 1:2000-4000 | — |
| Rat | — | — |
| Rabbit | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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