Histone H3 (Di Methyl Lys14) rabbit pAb from ELK Biotechnology Read more ›
Histone H3 (Di Methyl Lys14) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H3 in WB workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
| Specificity | The antibody detects endogenous Histone H3 (Di Methyl Lys14) protein. |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Rat Testis Tissue, diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
| Collaborator | ELK Biotechnology |
| Partner model | ES20556 |
| Target | Histone H3 |
| Research areas | >>Neutrophil extracellular trap formation, >>Alcoholism, >>Shigellosis, >>Transcriptional misregulation in cancer, >>Systemic lupus erythematosus |
| Reactivity | Human,Rat |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:500-1000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide of Histone H3 (Di Methyl Lys14) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-1000 | — |
| Rat | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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