Histone H4 (Di Methyl Lys79) rabbit pAb from ELK Biotechnology Read more ›
Histone H4 (Di Methyl Lys79) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H4 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],
| Specificity | The antibody detects endogenous Histone H4 (Di Methyl Lys79) protein. |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
| Collaborator | ELK Biotechnology |
| Partner model | ES20541 |
| Target | Histone H4 |
| Research areas | >>Neutrophil extracellular trap formation, >>Alcoholism, >>Viral carcinogenesis, >>Systemic lupus erythematosus |
| Reactivity | Human,Mouse,Rat |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:500-1000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide of Histone H4 (Di Methyl Lys79) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-1000 | — |
| Mouse | — | — |
| Rat | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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