Crk-L rabbit pAb from ELK Biotechnology Read more ›
Crk-L rabbit pAb is a research-use antibody from ELK Biotechnology for studying Crk-L in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],
| Specificity | Crk-L Polyclonal Antibody detects endogenous levels of Crk-L protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Crk-L Polyclonal Antibody | Immunofluorescence analysis of HUVEC cells, using CrkL Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CrkL Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from 293 cells, using CrkL Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2053 |
| Target | Crk-L |
| Research areas | >>MAPK signaling pathway, >>ErbB signaling pathway, >>Rap1 signaling pathway, >>Chemokine signaling pathway, >>Focal adhesion, >>Fc gamma R-mediated phagocytosis, >>Neurotrophin signaling pathway, >>Regulation of actin cytoskeleton, >>Insulin signaling pathway, >>Growth hormone synthesis, secretion and action, >>Bacterial invasion of epithelial cells, >>Shigellosis, >>Yersinia infection, >>Human cytomegalovirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Renal cell carcinoma, >>Chronic myeloid leukemia |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CrkL. AA range:173-222 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Skin,Spleen, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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