Connexin-26 rabbit pAb
Connexin-26 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Connexin-26 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Connexin-26.
Background
This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008],
Additional antibody information
| Specificity | Connexin-26 Polyclonal Antibody detects endogenous levels of Connexin-26 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Connexin-26 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysate from Jurkat cells, using Connexin-26 antibody. | Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using Connexin-26Rabbit pAb diluted at 1:200. |
Key Facts
| Target | Connexin-26 |
| Also known as | GJB2; Gap junction beta-2 protein; Connexin-26; Cx26 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 26kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . Cell junction, gap junction . Colocalizes with GJB4 at gap junction plaques in the cochlea. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Connexin-26. AA range:45-94 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.