Rac1/CDC42 (Phospho Ser71) rabbit pAb from ELK Biotechnology Read more ›
Rac1/CDC42 (Phospho Ser71) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Rac1/CDC42 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat Rac1/CDC42 (Phospho Ser71) |
|---|---|
| Validation evidence captions | Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rac1(Phospho-Ser71) antibody | Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES20202 |
| Target | Rac1/CDC42 |
| Research areas | >>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Phagosome, >>PI3K-Akt signaling pathway, >>Wnt signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>Natural killer cell mediated cytotoxicity, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Leukocyte transendothelial migration, >>Neurotrophin signaling pathway, >>Regulation of actin cytoskeleton, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabetic complications, >>Pancreatic secretion, >>Amyotrophic lateral sclerosis, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Bacterial invasion of epithelial cells, >>Epithelial cell signaling in Helicobacter pylori infect |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human Rac1/CDC42 (Phospho Ser71) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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