PPAR α (Phospho Ser21) rabbit pAb
PPAR α (Phospho Ser21) rabbit pAb from ELK Biotechnology Read more ›
Product Description
PPAR α (Phospho Ser21) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PPAR α.
Background
function:Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids.,online information:Peroxisome proliferator-activated receptor entry,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Heterodimer with the retinoid X receptor. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Also interacts with PPARBP coactivator in vitro. Interacts with AKAP13.,tissue specificity:Skeletal muscle, liver, heart and kidney.,
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human PPAR α (Phospho Ser21) |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | PPAR α |
| Also known as | Peroxisome proliferator-activated receptor alpha (PPAR-alpha;Nuclear receptor subfamily 1 group C member 1) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 52kD |
| Cellular localization | Nucleus. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>PPAR signaling pathway;>>cAMP signaling pathway;>>Adipocytokine signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Hepatitis C;>>Chemical carcinogenesis - receptor activation;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | Synthesized peptide derived from human PPAR α (Phospho Ser21) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.