Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96947

c-Jun (Phospho Ser249) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

c-Jun (Phospho Ser249) rabbit pAb from ELK Biotechnology Read more ›

c-Jun (Phospho Ser249) rabbit pAb
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c-Jun (Phospho Ser249) rabbit pAb

Product Description

c-Jun (Phospho Ser249) rabbit pAb is a research-use antibody from ELK Biotechnology for studying c-JUN in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.,PTM:Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer with either FOS or BATF3. Interacts with HIVEP3 (By similarity). Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.,

Additional antibody information

Specificity This antibody detects endogenous levels of Human,Mouse,Rat c-Jun (Phospho Ser249)
Validation evidence captions Western Blot analysis of various,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody Target: c-JUN
Collaborator ELK Biotechnology
Partner model ES20155
Target c-JUN
Research areas>>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>cAMP signaling pathway, >>Mitophagy - animal, >>Apoptosis, >>Wnt signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Tight junction, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabetic complications, >>Cocaine addiction, >>Amphetamine addiction, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Pertussis, >>Yersinia infection, >>Leishmaniasis, >>Chagas di
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human c-Jun (Phospho Ser249)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in the developing and adult prostate and prostate cancer cells.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000 ELISA 1:5000-20000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
c-JUN
Also known as
Transcription factor AP-1 (Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human c-Jun (Phospho Ser249)
Observed band
39-42kD
Cellular localization
Nucleus.
Dilution range
WB 1:1000-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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