RhoA (Phospho Ser188) rabbit pAb from ELK Biotechnology Read more ›
RhoA (Phospho Ser188) rabbit pAb is a research-use antibody from ELK Biotechnology for studying RhoA in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat RhoA (Phospho Ser188) |
|---|---|
| Validation evidence captions | Western Blot analysis of mouse kidney, rat kidney. Primary Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002 was diluted at 1:10000 | Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES20149 |
| Target | RhoA |
| Research areas | >>Ras signaling pathway, >>Rap1 signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Endocytosis, >>mTOR signaling pathway, >>Vascular smooth muscle contraction, >>Wnt signaling pathway, >>TGF-beta signaling pathway, >>Axon guidance, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Platelet activation, >>NOD-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>T cell receptor signaling pathway, >>Leukocyte transendothelial migration, >>Neurotrophin signaling pathway, >>Regulation of actin cytoskeleton, >>Oxytocin signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Pancreatic secretion, >>Bacterial invasion of epithelial cells, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Pertussis, >>Yersinia infection, >>Tuberculosis, >>Human cytomegalovirus infection, >>Pathways in cancer, >>Viral carcinogenesis, >>P |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human RhoA (Phospho Ser188) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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