ABM96944
BACE (Phospho Ser498) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
BACE (Phospho Ser498) rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
BACE (Phospho Ser498) rabbit pAb is an ELK Biotechnology antibody for research workflows involving BACE.
Background
beta-secretase 1(BACE1) Homo sapiens This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat BACE (Phospho Ser498) |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
Product type
Primary Antibody›
Target: BACE
| Target | BACE |
| Also known as | Beta-secretase 1 (EC 3.4.23.46;Aspartyl protease 2;ASP2;Asp 2;Beta-site amyloid precursor protein cleaving enzyme 1;Beta-site APP cleaving enzyme 1;Memapsin-2;Membrane-associated aspartic protease 2) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 ELISA 1:5000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 73kD |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein . Golgi apparatus, trans-Golgi network . Endoplasmic reticulum . Endosome . Cell surface . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Membrane raft . Lysosome . Late endosome . Early endosome . Recycling endosome . Cell projection, axon . Cell projection, dendrite . Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface (PubMed:17425515, PubMed:11466313). Colocalization with APP in early endosomes is due to addition of bisecting N-acetylglucosamine wich blocks targeting to late endosomes and lysosomes (By similarity). Retrogradly transported from end |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Alzheimer disease |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:1000-2000 ELISA 1:5000-20000 |
| Immunogen | Synthesized peptide derived from human BACE (Phospho Ser498) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
BACE
Also known as
Beta-secretase 1 (EC 3.4.23.46;Aspartyl protease 2;ASP2;Asp 2;Beta-site amyloid precursor protein cleaving enzyme 1;Beta-site APP cleaving enzyme 1;Memapsin-2;Membrane-associated aspartic protease 2)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human BACE (Phospho Ser498)
Observed band
73kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Golgi apparatus, trans-Golgi network . Endoplasmic reticulum . Endosome . Cell surface . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Membrane raft . Lysosome . Late endosome . Early endosome . Recycling endosome . Cell projection, axon . Cell projection, dendrite . Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface (PubMed:17425515, PubMed:11466313). Colocalization with APP in early endosomes is due to addition of bisecting N-acetylglucosamine wich blocks targeting to late endosomes and lysosomes (By similarity). Retrogradly transported from end
Dilution range
WB 1:1000-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed