FoxO1A (Acetyl Lys245) rabbit pAb
FoxO1A (Acetyl Lys245) rabbit pAb from ELK Biotechnology Read more ›
Product Description
FoxO1A (Acetyl Lys245) rabbit pAb is an ELK Biotechnology antibody for research workflows involving FoxO1.
Background
disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Interacts with LRPPRC.,tissue specificity:Ubiquitous.,
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat FoxO1A (Acetyl Lys245) |
|---|---|
| Validation evidence captions | Western Blot analysis of HEK 293 cells, cell treated or untreated by TSA 400nM 24h. Primary Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS23920 was diluted at 1:10000 |
Key Facts
| Target | FoxO1 |
| Also known as | Forkhead box protein O1 (Forkhead box protein O1A;Forkhead in rhabdomyosarcoma) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 ELISA 1:5000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 72kD |
| Cellular localization | Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Shigellosis;>>Human papillomavirus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Prostate cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:1000-2000 ELISA 1:5000-20000 |
| Immunogen | Synthesized peptide derived from human FoxO1A (Acetyl Lys245) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.