CIDE-B rabbit pAb from ELK Biotechnology Read more ›
CIDE-B rabbit pAb is a research-use antibody from ELK Biotechnology for studying CIDE-B in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Activates apoptosis.,similarity:Contains 1 CIDE-N domain.,subunit:Inhibited by DFFB. Interacts with DFFA and DFFB.,tissue specificity:Highly expressed in liver and small intestine and, at lower levels, in colon, kidney and spleen.,
| Specificity | CIDE-B Polyclonal Antibody detects endogenous levels of CIDE-B protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using CIDE-B Polyclonal Antibody | Immunofluorescence analysis of COS7 cells, using CIDEB Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human cervix caecinoma tissue, using CIDEB Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HT-29 and RAW264.7 cells, using CIDEB Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1983 |
| Target | CIDE-B |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CIDEB. AA range:91-140 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in liver and small intestine and, at lower levels, in colon, kidney and spleen. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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