Cdc23 rabbit pAb from ELK Biotechnology Read more ›
Cdc23 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cdc23 in WB, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008],
| Specificity | Cdc23 Polyclonal Antibody detects endogenous levels of Cdc23 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cdc23 Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of COS7 cells using Cdc23 Polyclonal Antibody diluted at 1:2000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES1925 |
| Target | Cdc23 |
| Research areas | >>Cell cycle, >>Oocyte meiosis, >>Ubiquitin mediated proteolysis, >>Progesterone-mediated oocyte maturation, >>Human T-cell leukemia virus 1 infection |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APC8. AA range:251-300 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Bone marrow,Brain,Duodenum,Epithelium,Pancreas,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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