Alix rabbit pAb
Alix rabbit pAb from ELK Biotechnology Read more ›
Product Description
Alix rabbit pAb is an ELK Biotechnology antibody for research workflows involving Alix.
Background
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human Alix |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour. |
Key Facts
| Target | Alix |
| Also known as | Programmed cell death 6-interacting protein (PDCD6-interacting protein;ALG-2-interacting protein 1;Hp95) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 85-100kD |
| Cellular localization | Cytoplasm, cytosol . Melanosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Secreted, extracellular exosome . Cell junction, tight junction . Midbody, Midbody ring . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 at centrosomes of non-dividing cells. Component of the actomyosin-tight junction complex (By similarity). PDCD6IP targeting to the midbody requires the interaction with CEP55 (PubMed:18641129). . |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Viral life cycle - HIV-1;>>Endocytosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Immunogen | Synthesized peptide derived from human Alix AA range: 410-490 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.