Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96767

ANGP2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ANGP2 rabbit pAb from ELK Biotechnology Read more ›

ANGP2 rabbit pAb
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ANGP2 rabbit pAb

Product Description

ANGP2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ANGP2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of human ANGP2
Validation evidence captions Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.

Key Facts

Product typePrimary Antibody Target: ANGP2
Collaborator ELK Biotechnology
Partner model ES18353
Target ANGP2
Research areas>>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>HIF-1 signaling pathway, >>PI3K-Akt signaling pathway, >>Kaposi sarcoma-associated herpesvirus infection
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human ANGP2
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Colon,Lung,Substantia nigra,Umbilical vein endothelial cell,
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ANGP2
Also known as
Angiopoietin-2 (ANG-2)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human ANGP2
Observed band
55kD
Cellular localization
Secreted .
Dilution range
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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