ABM96767
ANGP2 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ANGP2 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ANGP2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving ANGP2.
Background
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human ANGP2 |
|---|---|
| Validation evidence captions | Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour. |
Key Facts
Product type
Primary Antibody›
Target: ANGP2
| Target | ANGP2 |
| Also known as | Angiopoietin-2 (ANG-2) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 55kD |
| Cellular localization | Secreted . |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>HIF-1 signaling pathway;>>PI3K-Akt signaling pathway;>>Kaposi sarcoma-associated herpesvirus infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Immunogen | Synthesized peptide derived from human ANGP2 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
ANGP2
Also known as
Angiopoietin-2 (ANG-2)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human ANGP2
Observed band
55kD
Cellular localization
Secreted .
Dilution range
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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