Primary Antibody ISO 9001|ABMIUM Verified™
ABM96727

CAGE-1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CAGE-1 rabbit pAb from ELK Biotechnology Read more ›

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CAGE-1 rabbit pAb
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CAGE-1 rabbit pAb

Product Description

CAGE-1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CAGE-1.

Background

tissue specificity:Testis-specific expression in normal tissues, but wide expression among cancer tissues and cell lines.,

Additional antibody information

Specificity CAGE-1 Polyclonal Antibody detects endogenous levels of CAGE-1 protein.
Validation evidence captions Western Blot analysis of various cells using CAGE-1 Polyclonal Antibody diluted at 1:2000 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CAGE1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HT-29 and Jurkat cells, using CAGE1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using CAGE1 antibody.

Key Facts

Product type Primary Antibody Target: CAGE-1
Target CAGE-1
Also known as CAGE1; CTAG3; Cancer-associated gene 1 protein; CAGE-1; Cancer/testis antigen 3; CT3
Concentration 1 mg/ml
Working concentration Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Species reactivity Human;Rat;Mouse;
Observed band 90kD
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHCIFELISA
Dilution range Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen The antiserum was produced against synthesized peptide derived from human CAGE1. AA range:711-760
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CAGE-1
Also known as
CAGE1; CTAG3; Cancer-associated gene 1 protein; CAGE-1; Cancer/testis antigen 3; CT3
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CAGE1. AA range:711-760
Observed band
90kD
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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