Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96721

Cadherin 9 Antibody – Cadherin-9 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Cadherin-9 rabbit pAb from ELK Biotechnology Read more ›

Cadherin-9 rabbit pAb
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Cadherin-9 rabbit pAb

Product Description

Cadherin 9 Antibody for research use. Review the supplied specifications, applications and documentation before ordering.

Cadherin-9 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cadherin-9 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Cadherin-9 Polyclonal Antibody detects endogenous levels of Cadherin-9 protein.
Validation evidence captions Western Blot analysis of various cells using Cadherin-9 Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using CDH9 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDH9 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO cells, using CDH9 Antibody. The lane on the right is blocked with the synthesized peptide.

How to evaluate Cadherin 9 Antibody – Cadherin-9 rabbit pAb

Assess this research-use product against the requirements of your experimental workflow before ordering.

  • Confirm the listed species reactivity and experimental application.
  • Review clonality, host species, format, concentration and storage conditions.
  • Use lot-specific documentation and suitable positive and negative controls.

If a specification or current document is not shown, contact ABMIUM scientific support for assistance before use.

Key Facts

Product typePrimary Antibody Target: Cadherin-9
Collaborator ELK Biotechnology
Partner model ES1827
Target Cadherin-9
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CDH9. AA range:201-250
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system PCR rescued clones,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Cadherin-9
Also known as
CDH9; Cadherin-9
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CDH9. AA range:201-250
Observed band
89kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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