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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96690

ASTL rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ASTL rabbit pAb from ELK Biotechnology Read more ›

ASTL rabbit pAb
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ASTL rabbit pAb

Product Description

ASTL rabbit pAb is a research-use antibody from ELK Biotechnology for studying ASTL in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of ASTL at Human/Mouse
Validation evidence captions Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: ASTL
Collaborator ELK Biotechnology
Partner model ES18225
Target ASTL
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1：500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human ASTL AA range: 115-165
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1：500-2000 —
Mouse — —

Specifications

Target
ASTL
Concentration
1 mg/ml
Working concentration
WB 1：500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human ASTL AA range: 115-165
Cellular localization
Cytoplasm . Cell membrane . Cytoplasmic vesicle, secretory vesicle, Cortical granule . Probably exocytosed from cortical granules during post-fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles at postnatal (PN) day 3. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS) and occasionally on the oolemma in 2-cell through morulae stages. Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS). .
Dilution range
WB 1：500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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