AT2A3 rabbit pAb from ELK Biotechnology Read more ›
AT2A3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AT2A3 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
| Specificity | This antibody detects endogenous levels of AT2A3 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°over night |
| Collaborator | ELK Biotechnology |
| Partner model | ES18220 |
| Target | AT2A3 |
| Research areas | >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Cardiac muscle contraction, >>Adrenergic signaling in cardiomyocytes, >>Thyroid hormone signaling pathway, >>Pancreatic secretion, >>Alzheimer disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1：500-2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human AT2A3 AA range: 472-522 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic origin and also expressed in several cancer cell lines. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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