Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96680

ATD3A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ATD3A rabbit pAb from ELK Biotechnology Read more ›

ATD3A rabbit pAb
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ATD3A rabbit pAb

Product Description

ATD3A rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATD3A in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM, Sep 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of ATD3A at Human
Validation evidence captions Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: ATD3A
Collaborator ELK Biotechnology
Partner model ES18214
Target ATD3A
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human ATD3A AA range: 433-483
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Overexpressed in lung adenocarcinomas (at protein level).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse

Specifications

Target
ATD3A
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human ATD3A AA range: 433-483
Cellular localization
Mitochondrion inner membrane ; Single-pass membrane protein . Mitochondrion matrix, mitochondrion nucleoid . In the mitochondrial inner membrane, enriched in sites with the potential to form contacts with the outer membrane (PubMed:20349121, PubMed:20154147). The N-terminal domain interacts with the inner surface of the mitochondrial outer membrane and the C-terminal domain localizes in a specific matrix compartment, where it is associated with nucleoids (PubMed:18063578). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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