Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96641

BGAT rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

BGAT rabbit pAb from ELK Biotechnology Read more ›

BGAT rabbit pAb
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BGAT rabbit pAb

Product Description

BGAT rabbit pAb is a research-use antibody from ELK Biotechnology for studying BGAT in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of BGAT at Human/Mouse
Validation evidence captions Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: BGAT
Collaborator ELK Biotechnology
Partner model ES18059
Target BGAT
Research areas>>Glycosphingolipid biosynthesis - lacto and neolacto series, >>Metabolic pathways
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human BGAT AA range: 222-272
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Blood,Human adenocarcinoma,PCR rescued clones,Peripheral,Peripheral blood,Peripheral lymphocytes,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
BGAT
Also known as
Histo-blood group ABO system transferase (Fucosylglycoprotein 3-alpha-galactosyltransferase) (Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase) (Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase) (EC 2.4.1.40) (Glycoprotein-fucosylgalactoside alpha-galactosyltransferase) (EC 2.4.1.37) (Histo-blood group A transferase) (A transferase) (Histo-blood group B transferase) (B transferase) (NAGAT) Cleaved into: Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human BGAT AA range: 222-272
Observed band
38kD
Cellular localization
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.47% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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