Bim (phospho-Ser77) rabbit pAb
Bim (phospho-Ser77) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Bim (phospho-Ser77) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Bim.
Background
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human Mouse Bim (phospho-Ser77) |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | Bim |
| Also known as | Bcl-2-like protein 11 (Bcl2-L-11) (Bcl2-interacting mediator of cell death) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Mouse |
| Observed band | 22kD |
| Cellular localization | Endomembrane system ; Peripheral membrane protein . Associated with intracytoplasmic membranes. .; Isoform BimEL: Mitochondrion. Translocates from microtubules to mitochondria on loss of cell adherence.; Isoform BimL: Mitochondrion.; Isoform BimS: Mitochondrion.; Isoform Bim-alpha1: Mitochondrion. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>FoxO signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Non-alcoholic fatty liver disease;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Colorectal cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthesized phosho peptide around human Bim (Ser77) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.