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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96576

CALCOCO2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CALCOCO2 rabbit pAb from ELK Biotechnology Read more ›

CALCOCO2 rabbit pAb
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CALCOCO2 rabbit pAb

Product Description

CALCOCO2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CALCOCO2 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

Additional antibody information

Specificity This antibody detects endogenous levels of CALCOCO2 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: CALCOCO2
Collaborator ELK Biotechnology
Partner model ES17885
Target CALCOCO2
Research areas>>Mitophagy - animal, >>Shigellosis, >>Influenza A
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human CALCOCO2
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Mouse — —
Rat — —

Specifications

Target
CALCOCO2
Also known as
Calcium-binding and coiled-coil domain-containing protein 2 (Antigen nuclear dot 52 kDa protein) (Nuclear domain 10 protein NDP52) (Nuclear domain 10 protein 52) (Nuclear dot protein 52)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human CALCOCO2
Observed band
53kD
Cellular localization
Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton . Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein . According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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