Caspase 10 (p23/17, Cleaved-Val220) Rabbit pAb from ELK Biotechnology Read more ›
Caspase 10 (p23/17, Cleaved-Val220) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Caspase-10 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011],
| Specificity | This antibody detects endogenous levels of human Caspase 10 (p23/17, Cleaved-Val220) |
|---|---|
| Validation evidence captions | Western blot analysis of Hela cell treated or untreated with 2 mM/ml camptothecin , primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour. |
| Collaborator | ELK Biotechnology |
| Partner model | ES17852 |
| Target | Caspase-10 |
| Research areas | >>Apoptosis, >>RIG-I-like receptor signaling pathway, >>TNF signaling pathway, >>Tuberculosis, >>Hepatitis B |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human Caspase 10 (p23/17, Cleaved-Val220) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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