Caspase 10 (p23/17, Cleaved-Val220) Rabbit pAb
Caspase 10 (p23/17, Cleaved-Val220) Rabbit pAb from ELK Biotechnology Read more ›
Product Description
Caspase 10 (p23/17, Cleaved-Val220) Rabbit pAb is an ELK Biotechnology antibody for research workflows involving Caspase-10.
Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human Caspase 10 (p23/17, Cleaved-Val220) |
|---|---|
| Validation evidence captions | Western blot analysis of Hela cell treated or untreated with 2 mM/ml camptothecin , primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour. |
Key Facts
| Target | Caspase-10 |
| Also known as | Caspase-10 (CASP-10;EC 3.4.22.63;Apoptotic protease Mch-4;FAS-associated death domain protein interleukin-1B-converting enzyme 2;FLICE2;ICE-like apoptotic protease 4) Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Species reactivity | Human; Mouse; Rat |
| Observed band | 58+23kD |
| Cellular localization | cytosol,CD95 death-inducing signaling complex,ripoptosome, |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Apoptosis;>>RIG-I-like receptor signaling pathway;>>TNF signaling pathway;>>Tuberculosis;>>Hepatitis B |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA(peptide)1:5000-20000 |
| Immunogen | Synthesized peptide derived from human Caspase 10 (p23/17, Cleaved-Val220) |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.