c-Cbl (phospho-Tyr731) rabbit pAb from ELK Biotechnology Read more ›
c-Cbl (phospho-Tyr731) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cbl in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Cbl proto-oncogene(CBL) Homo sapiens This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016],
| Specificity | This antibody detects endogenous levels of Human c-Cbl (phospho-Tyr731) |
|---|---|
| Validation evidence captions | Western Blot analysis of 1 K562 treated with LPS, 2 K562,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES17789 |
| Target | Cbl |
| Research areas | >>ErbB signaling pathway, >>Ubiquitin mediated proteolysis, >>Endocytosis, >>Insulin signaling pathway, >>Bacterial invasion of epithelial cells, >>Pathways in cancer, >>Proteoglycans in cancer, >>Chronic myeloid leukemia |
| Reactivity | Human,Rat,Mouse |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1000-2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phosho peptide around human c-Cbl (Tyr731) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Epithelium,T-cell, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Rat | — | — |
| Mouse | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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