Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96492

CCL17 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CCL17 rabbit pAb from ELK Biotechnology Read more ›

CCL17 rabbit pAb
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CCL17 rabbit pAb

Product Description

CCL17 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CCL17 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. [provided by RefSeq, Sep 2014],

Additional antibody information

Specificity This antibody detects endogenous levels of human CCL17
Validation evidence captions Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.

Key Facts

Product typePrimary Antibody Target: CCL17
Collaborator ELK Biotechnology
Partner model ES17761
Target CCL17
Research areas>>Cytokine-cytokine receptor interaction, >>Viral protein interaction with cytokine and cytokine receptor, >>Chemokine signaling pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human CCL17 AA range: 21-70
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CCL17
Also known as
C-C motif chemokine 17 (CC chemokine TARC;Small-inducible cytokine A17;Thymus and activation-regulated chemokine)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human CCL17 AA range: 21-70
Observed band
10kD
Cellular localization
Secreted.
Dilution range
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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