Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96479

BCAS2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

BCAS2 rabbit pAb from ELK Biotechnology Read more ›

BCAS2 rabbit pAb
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BCAS2 rabbit pAb

Product Description

BCAS2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying BCAS2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Involved in mRNA splicing.,similarity:Belongs to the SPF27 family.,subunit:Associated with the spliceosome.,tissue specificity:Ubiquitously expressed.,

Additional antibody information

Specificity BCAS2 Polyclonal Antibody detects endogenous levels of BCAS2 protein.
Validation evidence captions Western Blot analysis of various cells using BCAS2 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western Blot analysis of COS7 cells using BCAS2 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of the lysates from HUVECcells using BCAS2 antibody.

Key Facts

Product typePrimary Antibody Target: BCAS2
Collaborator ELK Biotechnology
Partner model ES1755
Target BCAS2
Research areas>>Spliceosome
Reactivity Human,Mouse,Rat,Monkey
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human BCAS2. AA range:176-225
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitously expressed.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
BCAS2
Also known as
BCAS2; DAM1; Pre-mRNA-splicing factor SPF27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human BCAS2. AA range:176-225
Observed band
27kD
Cellular localization
Nucleus . Nucleus, nucleolus .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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