ABM96462
CEP85 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CEP85 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CEP85 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CEP85.
Background
This gene encodes a protein that belongs to the centrosome-associated family of proteins. The centrosome is a subcellular organelle in the animal cell that functions as a microtubule organizing center and is involved in cell-cycle progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
Additional antibody information
| Specificity | This antibody detects endogenous levels of CEP85 at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: CEP85
| Target | CEP85 |
| Also known as | Centrosomal protein of 85 kDa (Coiled-coil domain-containing protein 21) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse |
| Observed band | 85kD |
| Cellular localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Nucleus, nucleolus . Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles (PubMed:21399614). Associated with the pericentriolar material. Localizes to centrosomes at a low level in G1 phase and a slightly increased level in S phase, with gradually elevated levels during G2 phase. The levels at centrosomes further increase at G2/M, reaching a peak at spindle poles at early mitotic stages and remain high until the end of anaphase (PubMed:26220856). . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.295% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human CEP85 AA range: 267-317 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
Specifications
Target
CEP85
Also known as
Centrosomal protein of 85 kDa (Coiled-coil domain-containing protein 21)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.295% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human CEP85 AA range: 267-317
Observed band
85kD
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Nucleus, nucleolus . Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles (PubMed:21399614). Associated with the pericentriolar material. Localizes to centrosomes at a low level in G1 phase and a slightly increased level in S phase, with gradually elevated levels during G2 phase. The levels at centrosomes further increase at G2/M, reaching a peak at spindle poles at early mitotic stages and remain high until the end of anaphase (PubMed:26220856). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.295% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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