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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96462

CEP85 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CEP85 rabbit pAb from ELK Biotechnology Read more ›

CEP85 rabbit pAb
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CEP85 rabbit pAb

Product Description

CEP85 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CEP85 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein that belongs to the centrosome-associated family of proteins. The centrosome is a subcellular organelle in the animal cell that functions as a microtubule organizing center and is involved in cell-cycle progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Additional antibody information

Specificity This antibody detects endogenous levels of CEP85 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: CEP85
Collaborator ELK Biotechnology
Partner model ES17522
Target CEP85
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human CEP85 AA range: 267-317
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Hepatoma,Placenta,Retinoblastoma,Testis,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Mouse — —

Specifications

Target
CEP85
Also known as
Centrosomal protein of 85 kDa (Coiled-coil domain-containing protein 21)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human CEP85 AA range: 267-317
Observed band
85kD
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Nucleus, nucleolus . Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles (PubMed:21399614). Associated with the pericentriolar material. Localizes to centrosomes at a low level in G1 phase and a slightly increased level in S phase, with gradually elevated levels during G2 phase. The levels at centrosomes further increase at G2/M, reaching a peak at spindle poles at early mitotic stages and remain high until the end of anaphase (PubMed:26220856). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.295% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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