Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96436

CHCHD4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CHCHD4 rabbit pAb from ELK Biotechnology Read more ›

CHCHD4 rabbit pAb
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CHCHD4 rabbit pAb

Product Description

CHCHD4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CHCHD4 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of CHCHD4 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: CHCHD4
Collaborator ELK Biotechnology
Partner model ES17486
Target CHCHD4
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human CHCHD4 AA range: 35-85
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in all tissues tested, suggesting an ubiquitous expression.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat

Specifications

Target
CHCHD4
Also known as
Mitochondrial intermembrane space import and assembly protein 40 (Coiled-coil-helix-coiled-coil-helix domain-containing protein 4)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human CHCHD4 AA range: 35-85
Observed band
22kD
Cellular localization
Mitochondrion intermembrane space .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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