Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96385

CLH2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CLH2 rabbit pAb from ELK Biotechnology Read more ›

CLH2 rabbit pAb
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CLH2 rabbit pAb

Product Description

CLH2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CLH2 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of the clathrin heavy chain family and encodes a major protein of the polyhedral coat of coated pits and vesicles. Chromosomal aberrations involving this gene are associated with meningioma, DiGeorge syndrome, and velo-cardio-facial syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of CLH2 at Human
Validation evidence captions Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: CLH2
Collaborator ELK Biotechnology
Partner model ES17375
Target CLH2
Research areas>>Lysosome, >>Endocytosis, >>Synaptic vesicle cycle, >>Endocrine and other factor-regulated calcium reabsorption, >>Huntington disease, >>Bacterial invasion of epithelial cells
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human CLH2 AA range: 893-943
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse

Specifications

Target
CLH2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human CLH2 AA range: 893-943
Cellular localization
Cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane, coated pit ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic face of coated pits and vesicles. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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