COX82 Rabbit pAb
COX82 Rabbit pAb from ELK Biotechnology Read more ›
Product Description
COX82 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving COX82.
Background
The protein encoded by this gene is the terminal enzyme of the respiratory chain, coupling the transfer of electrons from cytochrome c to molecular oxygen, with the concomitant production of a proton electrochemical gradient across the inner mitochondrial membrane. In addition to 3 mitochondrially encoded subunits, which perform the catalytic function, the eukaryotic enzyme contains nuclear-encoded smaller subunits, ranging in number from 4 in some organisms to 10 in mammals. It has been proposed that nuclear-encoded subunits may be involved in the modulation of the catalytic function. This gene encodes one of the nuclear-encoded subunits. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human COX82 |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
Key Facts
| Target | COX82 |
| Also known as | Cytochrome c oxidase subunit 8A, mitochondrial (Cytochrome c oxidase polypeptide VIII-liver/heart;Cytochrome c oxidase subunit 8-2) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Cellular localization | Mitochondrion inner membrane ; Single-pass membrane protein . |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Oxidative phosphorylation;>>Metabolic pathways;>>Cardiac muscle contraction;>>Thermogenesis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCELISA |
| Dilution range | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Immunogen | Synthesized peptide derived from human COX82 AA range: 10-90 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.