Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96243

Arrdc1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Arrdc1 rabbit pAb from ELK Biotechnology Read more ›

Arrdc1 rabbit pAb
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Arrdc1 rabbit pAb

Product Description

Arrdc1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Arrdc1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

similarity:Belongs to the arrestin family.,

Additional antibody information

Specificity Arrdc1 Polyclonal Antibody detects endogenous levels of Arrdc1 protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of various cells using Arrdc1 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of Jurkat cells using Arrdc1 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from Jurkat cells, using ARRD1 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: Arrdc1
Collaborator ELK Biotechnology
Partner model ES1708
Target Arrdc1
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ARRD1. AA range:167-216
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Blood,Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Arrdc1
Also known as
ARRDC1; Arrestin domain-containing protein 1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ARRD1. AA range:167-216
Observed band
48kD
Cellular localization
Cell membrane . Also found in extracellular vesicles different from exosomes. .
Dilution range
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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