Arp3 rabbit pAb from ELK Biotechnology Read more ›
Arp3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Arp3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013],
| Specificity | Arp3 Polyclonal Antibody detects endogenous levels of Arp3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Arp3 Polyclonal Antibody | Western Blot analysis of K562 cells using Arp3 Polyclonal Antibody | Western blot analysis of lysates from Jurkat and RAW264.7 cells, using ACTR3 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using ACTR3 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1707 |
| Target | Arp3 |
| Research areas | >>Endocytosis, >>Tight junction, >>Fc gamma R-mediated phagocytosis, >>Regulation of actin cytoskeleton, >>Bacterial invasion of epithelial cells, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Yersinia infection |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACTR3. AA range:369-418 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Cajal-Retzius cell,T-cell, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
