Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96233

DARPP-32 (phospho-Ser97) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

DARPP-32 (phospho-Ser97) rabbit pAb from ELK Biotechnology Read more ›

DARPP-32 (phospho-Ser97) rabbit pAb
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DARPP-32 (phospho-Ser97) rabbit pAb

Product Description

DARPP-32 (phospho-Ser97) rabbit pAb is a research-use antibody from ELK Biotechnology for studying DARPP-32 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Mouse Rat DARPP-32 (phospho-Ser97)
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: DARPP-32
Collaborator ELK Biotechnology
Partner model ES17020
Target DARPP-32
Research areas>>cAMP signaling pathway, >>Dopaminergic synapse, >>Cocaine addiction, >>Amphetamine addiction, >>Alcoholism
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human DARPP-32 (Ser97)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Adipose tissue,Brain,Cerebellum,Colon,Ovary,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Mouse
Rat

Specifications

Target
DARPP-32
Also known as
Protein phosphatase 1 regulatory subunit 1B (DARPP-32) (Dopamine- and cAMP-regulated neuronal phosphoprotein)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized phosho peptide around human DARPP-32 (Ser97)
Observed band
30kD
Cellular localization
Cytoplasm.
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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