ABM96233
DARPP-32 (phospho-Ser97) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
DARPP-32 (phospho-Ser97) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
DARPP-32 (phospho-Ser97) rabbit pAb is an ELK Biotechnology antibody for research workflows involving DARPP-32.
Background
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human Mouse Rat DARPP-32 (phospho-Ser97) |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: DARPP-32
| Target | DARPP-32 |
| Also known as | Protein phosphatase 1 regulatory subunit 1B (DARPP-32) (Dopamine- and cAMP-regulated neuronal phosphoprotein) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 30kD |
| Cellular localization | Cytoplasm. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>cAMP signaling pathway;>>Dopaminergic synapse;>>Cocaine addiction;>>Amphetamine addiction;>>Alcoholism |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthesized phosho peptide around human DARPP-32 (Ser97) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Target
DARPP-32
Also known as
Protein phosphatase 1 regulatory subunit 1B (DARPP-32) (Dopamine- and cAMP-regulated neuronal phosphoprotein)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WB
Immunogen
Synthesized phosho peptide around human DARPP-32 (Ser97)
Observed band
30kD
Cellular localization
Cytoplasm.
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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