ABM96120
AQP12 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
AQP12 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
AQP12 rabbit pAb is an ELK Biotechnology antibody for research workflows involving AQP12.
Background
domain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,function:Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily.,tissue specificity:Restricted to the pancreas.,
Additional antibody information
| Specificity | AQP12 Polyclonal Antibody detects endogenous levels of AQP12 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using AQP12 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from Jurkat cells, using AQP12 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using AQP12 antibody. |
Key Facts
Product type
Primary Antibody›
Target: AQP12
| Target | AQP12 |
| Also known as | AQP12A; AQP12; AQPX2; Aquaporin-12A; AQP-12; AQP12B; Aquaporin-12B; AQP-12B |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 34kD |
| Cellular localization | Membrane ; Multi-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AQP12. AA range:231-280 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
AQP12
Also known as
AQP12A; AQP12; AQPX2; Aquaporin-12A; AQP-12; AQP12B; Aquaporin-12B; AQP-12B
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human AQP12. AA range:231-280
Observed band
34kD
Cellular localization
Membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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