AQP12 rabbit pAb from ELK Biotechnology Read more ›
AQP12 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AQP12 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
domain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,function:Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily.,tissue specificity:Restricted to the pancreas.,
| Specificity | AQP12 Polyclonal Antibody detects endogenous levels of AQP12 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using AQP12 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from Jurkat cells, using AQP12 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using AQP12 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1687 |
| Target | AQP12 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AQP12. AA range:231-280 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Restricted to the pancreas. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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