APPL1 rabbit pAb from ELK Biotechnology Read more ›
APPL1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying APPL1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1(APPL1) Homo sapiens The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008],
| Specificity | APPL1 Polyclonal Antibody detects endogenous levels of APPL1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using APPL1 Polyclonal Antibody | Western blot analysis of the lysates from K562 cells using APPL1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1684 |
| Target | APPL1 |
| Research areas | >>Longevity regulating pathway, >>Pathways in cancer, >>Colorectal cancer |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APPL1. AA range:121-170 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | High levels in heart, ovary, pancreas and skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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