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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96113

DZIP1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

DZIP1 rabbit pAb from ELK Biotechnology Read more ›

DZIP1 rabbit pAb
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DZIP1 rabbit pAb

Product Description

DZIP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying DZIP1 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of DZIP1 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: DZIP1
Collaborator ELK Biotechnology
Partner model ES16834
Target DZIP1
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1：500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human DZIP1 AA range: 73-123
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Predominantly expressed in testis (at protein level) (PubMed:15081113, PubMed:32051257). Also expressed in fetal brain, adult oocytes and ovary (PubMed:15081113). Expressed in undifferentiated ES cells (PubMed:15081113). In testis, it is specifically expressed in germ cells (at protein level) (PubMed:15081113, PubMed:32051257). Expressed in mature germ cells and secondary spermatocytes, while it is weakly or not expressed in primary spermatocytes (PubMed:15081113).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1：500-2000 —
Mouse — —

Specifications

Target
DZIP1
Concentration
1 mg/ml
Working concentration
WB 1：500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human DZIP1 AA range: 73-123
Cellular localization
Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Nucleus . Nucleus speckle . Cytoplasm . Localizes to the centriole in cells lacking cilia and to the cilium basal body in ciliated cells (PubMed:19852954). At the exit of mitosis, when the primary cilium is reassembled in daughter cells, localizes at the mother centriole that acts as the basal body of the assembling primary cilium and also accumulates at the ciliary base that constitutes a diffusion barrier for ciliary proteins (By similarity). .
Dilution range
WB 1：500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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