ECHA rabbit pAb
ECHA rabbit pAb from ELK Biotechnology Read more ›
Product Description
ECHA rabbit pAb is an ELK Biotechnology antibody for research workflows involving ECHA.
Background
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of ECHA at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | ECHA |
| Also known as | Trifunctional enzyme subunit alpha, mitochondrial (78 kDa gastrin-binding protein) (TP-alpha) Includes: Long-chain enoyl-CoA hydratase (EC 4.2.1.17); Long chain 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 85kD |
| Cellular localization | Mitochondrion . Mitochondrion inner membrane . Protein stability and association with mitochondrion inner membrane do not require HADHB. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.301% sodium azide. |
| Research area | >>Fatty acid elongation;>>Fatty acid degradation;>>Valine, leucine and isoleucine degradation;>>Lysine degradation;>>Tryptophan metabolism;>>beta-Alanine metabolism;>>Propanoate metabolism;>>Butanoate metabolism;>>Metabolic pathways;>>Fatty acid metabolism |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human ECHA AA range: 276-326 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.