Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96097

EGF Receptor (phospho-Tyr1148) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

EGF Receptor (phospho-Tyr1148) rabbit pAb from ELK Biotechnology Read more ›

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Product Description

EGF Receptor (phospho-Tyr1148) rabbit pAb is a research-use antibody from ELK Biotechnology for studying EGFR in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of Human EGF Receptor (phospho-Tyr1148)

Key Facts

Product typePrimary Antibody Target: EGFR
Collaborator ELK Biotechnology
Partner model ES16790
Target EGFR
Research areas>>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Phospholipase D signaling pathway, >>Endocytosis, >>PI3K-Akt signaling pathway, >>Focal adhesion, >>Adherens junction, >>Gap junction, >>JAK-STAT signaling pathway, >>Regulation of actin cytoskeleton, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Cushing syndrome, >>Epithelial cell signaling in Helicobacter pylori infection, >>Shigellosis, >>Hepatitis C, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Chemical carcinogenesis - reactive oxygen species, >>Colorectal cance
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human EGF Receptor (Tyr1148)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Rat
Mouse

Specifications

Target
EGFR
Also known as
Epidermal growth factor receptor (EC 2.7.10.1) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized phosho peptide around human EGF Receptor (Tyr1148)
Observed band
134kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). .; Isoform 2: Secreted.
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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