APHC rabbit pAb from ELK Biotechnology Read more ›
APHC rabbit pAb is a research-use antibody from ELK Biotechnology for studying APHC in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta.,
| Specificity | APHC Polyclonal Antibody detects endogenous levels of APHC protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using APHC Polyclonal Antibody | Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO cells, using PHCA Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1677 |
| Target | APHC |
| Research areas | >>Sphingolipid metabolism, >>Metabolic pathways |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed. Highly expressed in placenta (PubMed:11356846). Expressed in erythrocytes (PubMed:20207939). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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