Primary Antibody ISO 9001|ABMIUM Verified™
ABM96088

eIF5A/eIF5A2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

eIF5A/eIF5A2 rabbit pAb from ELK Biotechnology Read more ›

Related formats
eIF5A/eIF5A2 rabbit pAb
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eIF5A/eIF5A2 rabbit pAb

Product Description

eIF5A/eIF5A2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving eIF5A/eIF5A2.

Additional antibody information

Specificity This antibody detects endogenous levels of human eIF5A/eIF5A2
Validation evidence captions Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product type Primary Antibody Target: eIF5A/eIF5A2
Target eIF5A/eIF5A2
Also known as Eukaryotic translation initiation factor 5A-1 (eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D)
Concentration 1 mg/ml
Working concentration WB 1:500-2000, ELISA(peptide)1:5000-20000
Species reactivity Human; Mouse; Rat
Observed band 17kD
Cellular localization Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBELISA
Dilution range WB 1:500-2000, ELISA(peptide)1:5000-20000
Immunogen Synthesized peptide derived from human eIF5A/eIF5A2
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
eIF5A/eIF5A2
Also known as
Eukaryotic translation initiation factor 5A-1 (eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse; Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human eIF5A/eIF5A2
Observed band
17kD
Cellular localization
Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .
Dilution range
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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